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KMID : 0383820130750050205
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Tuberculosis and Respiratory Diseases
2013 Volume.75 No. 5 p.205 ~ p.209
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Effect of Prunetin on TNF-α-Induced MUC5AC Mucin Gene Expression, Production, Degradation of IkB and Translocation of NF-κB p65 in Human Airway Epithelial Cells
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Ryu Ji-Ho
Lee Hyun-Jae
Park Su-Hyun
Md. Asaduzzaman Sikder
Kim Ju-Ock
Hong Jang-Hee
Seok Jeong-Ho
Lee Choong-Jae
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Abstract
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Background: We investigated whether prunetin significantly affects tumor necrosis factor-α (TNF-α)-induced MUC5AC mucin gene expression, production, inhibitory kappa B (IkB) degradation and nuclear factor kappa B (NF-kB) p65 translocation in human airway epithelial cells.
Materials and Methods: Confluent NCI-H292 cells were pretreated with prunetin for 30 minutes and then stimulated with TNF-α for 24 hours or the indicated periods. MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. The effect of prunetin on TNF-α-induced degradation of IkB and translocation of NF-κB p65 was investigated by western blot analysis.
Results: We found that incubation of NCI-H292 cells with prunetin significantly inhibited mucin production and down-regulated the MUC5AC gene expression induced by TNF-α. Prunetin inhibited TNF-α-induced degradation of IkB and translocation of NF-κB p65.
Conclusion: This result suggests that prunetin inhibits the NF-κB signaling pathway, which may explain its role in the inhibition of MUC5AC mucin gene expression and production regulated by the NF-κB signaling pathway.
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KEYWORD
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Mucins, MUC5AC Protein, Human, Prunetin
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